For Atlantic salmon, 472 miRNAs have been reported in the miRBase. Different biological process, such as development, growth, cell division, metabolism, and apoptosis, are regulated by miRNAs. This study is the first to evidence the interplaying among mRNAs and miRNAs and the structural relationship at the genome level during Atlantic salmon smoltification.Īmong ncRNAs, microRNAs (miRNAs) are crucial in post-transcriptional regulation, binding to target mRNAs in 3′UTR and repressing translation to proteins. However, target analysis of these miRNAs showed a regulatory role in growth, stress response, and immunity. miRNA expression analysis suggested a small number of miRNAs involved in the smoltification process. In addition, differential expression analysis showed a greater number of transcripts modulated in response to SS in gills and head kidney. Chromosome regions highly expressed between experimental conditions included a great abundance of transposable elements. Different expression patterns among the tissues and treatments were observed through a whole-genome transcriptomic approach. Gills, intestine, and head kidney samples were used for total RNA extraction, followed by mRNA and small RNA illumina sequencing. Atlantic salmon smolts were transferred to seawater under two strategies: (i) fish group exposed to gradual salinity changes (GSC) and (ii) fish group exposed to a salinity shock (SS). This study proposes a novel approach to analyze complex data sets combining transcriptomes and miRNAs at the chromosome-level genome. However, analysis of complex transcriptomic experimental designs involving different conditions, tissues, or times is relevant. The growing amount of genome information and transcriptomes data available allows for a better understanding of biological processes. The findings uncover whole-transcriptome modulation during seawater adaptation of Atlantic salmon, evidencing the interplaying among mRNAs and miRNAs. However, target analysis of these miRNAs suggests a regulatory role of process such as growth, stress response, and immunity. Moreover, small RNA expression analysis suggested fewer of miRNAs associated with the smoltification process. A great abundance of transposable elements was observed in chromosome regions differentially expressed under experimental conditions. The whole-genome transcript expression profiling revealed specific gene expression patterns among the tissues and treatments. mRNA and miRNAs sequencing were performed for gills, intestine, and head kidney tissues. Salmon salar smolts were transferred to seawater under two strategies: (i) fish group exposed to gradual salinity changes (GSC) and (ii) fish group exposed to a salinity shock (SS). This study proposes a novel approach to analyze transcriptome data sets using the Atlantic salmon seawater adaptation process as a model.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |